Preapproved Chemicals


The following chemicals are permitted in the BUGSS facility as long as they are in their original labeled container, specifying the identity, concentration, hazard rating and storage conditions. A MSDS must accompany the chemical and will be placed in the BUGSS MSDS file. For chemicals not on this white list specific written permission is required from the BUGSS safety officer ([email protected]) before the chemical can be allowed into the facility.

Tryptone

Yeast Extract

Peptone

Amino Acids

L-proline

Vitamins

Agar

Glucose

Sucrose

Galactose

Arabinose

Raffinose

Agarose

Tris-HCL

Tris Base

Sodium acetate

Sodium chloride

Calcium Chloride

Ammonium chloride

Magnesium chloride

Magnesium sulfate

Gel-Red

Brome phenol Blue

Glycerol

Triton X-100

Tween 20

IGEPAL®CA-630

Ethanol (quantity limited)

Isopropanol (quantity limited)

DMSO (quantity limited)

IPTG

Ampicillin

Kanamycin

Chloramphenicol

Tetracycline

Lithium acetate

Potassium Chloride

Acetic Acid (quantity limited)

HCL (quantity limited)

NaOH (quantity limited)

Lysozyme

RNase A

Restriction and other DNA modifying enzymes

Zymolyase

Calf Thymus DNA

Anti-mouse IgG

BCIP®/NBT solution

EDTA

Murashige & Skoog Basal medium

Murashige & Skoog Basal  salts with minimal organics

Sodium dodecyl sulfate

Tryptic soy broth

D-mannitol

10% FeCl3

Instant Ocean

PEG-3350

Sephadex G-50

Lactose

Potassium phosphate monobasic

Potassium phosphate dibasic

Yeast nitrogen base w/ ammonium sulfate w/o amino acids

Simply Blue Safe Stain

Tris-Glycine running buffer 10X

Gram stain kit w/ grams iodine, crystal violet, saffron red

Bromophenol blue

ZnCl2 1M solution

Kinetin 1mg/ml

Alpha-Naphthaleneacetic acid 1mg/ml

 Preapproved Organisms


The following organisms are permitted in the BUGSS facility as long as they are accompanied by documentation attesting to their identity and source. For organisms not on this white list specific written permission is required from the BUGSS safety officer ([email protected]) before the organism can be allowed into the facility. This white list is a compilation taken from the WHO laboratory safety manual 3rd Edition, the NIH/CDC Biosafety in Biomedical research facilities and Biological Safety Principals and Practices 4th Edition.

Prokaryotic Microbes:

Escherichia coli K-12 strains and derivatives:

JM109

DH5α

HB101

MC1066

MM294

Top 10 F’

MG1655

Escherichia coli B strains and derivatives:

BL21 (DE3)

Bacillus subtilus

Thermus aquaticus

Thermus thermophilus

Geobacillus stearothermophilus

Ralstonia metallidurans

Eukaryotic microbes:

Saccharomyces cerevisiae S288C strains and derivatives:

Pichia pastoris strains and derivatives

Mammalian cells:

CHO (Chinese hamster ovary) cells and derivatives

Plant Cells:

Arabidopsis thaliana and derivatives

Protozoa:

Pyrocystis fusiformis